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apoptotic dna ladder extraction kit  (Beyotime)


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    Beyotime apoptotic dna ladder extraction kit
    Apoptotic Dna Ladder Extraction Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apoptotic+dna+ladder+kit/DNA+Ladder/pm39154542-91-6-12
    Average 99 stars, based on 267 article reviews
    apoptotic dna ladder extraction kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Purification:

    Article Title: microRNA let-7c is essential for the anisomycin-elicited apoptosis in Jurkat T cells by linking JNK1/2 to AP-1/STAT1/STAT3 signaling.
    Article Snippet: .. After the treatments, their fragmented DNAs were extracted and purified using Apoptotic DNA Ladder Kit (Beyotime, Shanghai, China) according to the manufacturer’s instructions. ..

    Agarose Gel Electrophoresis:

    Article Title: Evaluating the cytotoxicity mechanism of the cell-penetrating peptide TP10 on Jurkat cells.
    Article Snippet: TP10, a classic cell-penetrating peptide, shows a high degree of similarity to AMPs in structure.. Although TP10 has been widely used in drug delivery, the mechanism underlying its cytotoxicity is yet to be elucidated.. Herein, we explored the cell-killing mechanism of TP10 against human leukemia Jurkat cells.



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    EHEC O157 OMVs administered to mice by oral gavage cause tubular epithelial damage and apoptosis. Histopathology of paraffin sections stained with hematoxylin–eosin (A–C) and transmission electron microscopy (D–F) of the kidneys from mice treated with 100–400 µg of OMVs (A, B, D, E) and from PBS-treated mice (C, F) . (A) Tubular epithelial cell flattening (black arrows) and intraluminal detached epithelial cells (asterisks). (B) Tubular epithelial flattening (black arrows), extensive vacuolization (blue arrows), detached intraluminal epithelial cells (asterisks). (C) Normal tubules (arrows) and glomeruli in a PBS-treated mouse. (D) Microvacuolization (thin white arrows), macrovacuolization (thick white arrows), and flattening of tubular epithelial cells with a preserved brush border (white asterisks). (E) Macrovacuolization of tubular epithelial cells (red arrows) with lost brush border; the green arrow indicates an <t>apoptotic</t> nucleus. (F) Normal tubule in a PBS-treated mouse; the yellow arrow indicates a normal tubular epithelial cell and the white asterisk a preserved brush border. (A–C) Magnification 400x; (D–F) scale bars, 5 µm. Images are representative of findings in mice treated with 100 µg, 200 µg, or 400 µg of EHEC O157 OMVs or PBS. (G) Pathology score for tubular epithelial damage was assessed based on the extent of brush border loss, epithelial cell flattening, and vacuolization after 72 h. Data are means ± standard deviations for groups of mice administered the indicated OMV dose or PBS; **** p < 0.0001 for mice treated with 100–400 µg of OMVs compared with mice treated with PBS (one-way ANOVA). (H) Apoptosis shown as <t>DNA</t> laddering in the kidneys of mice treated with 100 µg (lane 2), 200 µg (lane 3), or 400 µg (lane 4) of OMVs for 72 h Lanes 1 and 5, kidneys from mice treated with 5 µg and 25 µg of OMVs, respectively. Lanes 6 and 7, kidneys from mice treated with PBS. Lane 8, kidney treated with 1 µM staurosporine (positive control). Lanes M, molecular size marker (100 bp ladder). The entire original gel is shown in .
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    EHEC O157 OMVs administered to mice by oral gavage cause tubular epithelial damage and apoptosis. Histopathology of paraffin sections stained with hematoxylin–eosin (A–C) and transmission electron microscopy (D–F) of the kidneys from mice treated with 100–400 µg of OMVs (A, B, D, E) and from PBS-treated mice (C, F) . (A) Tubular epithelial cell flattening (black arrows) and intraluminal detached epithelial cells (asterisks). (B) Tubular epithelial flattening (black arrows), extensive vacuolization (blue arrows), detached intraluminal epithelial cells (asterisks). (C) Normal tubules (arrows) and glomeruli in a PBS-treated mouse. (D) Microvacuolization (thin white arrows), macrovacuolization (thick white arrows), and flattening of tubular epithelial cells with a preserved brush border (white asterisks). (E) Macrovacuolization of tubular epithelial cells (red arrows) with lost brush border; the green arrow indicates an <t>apoptotic</t> nucleus. (F) Normal tubule in a PBS-treated mouse; the yellow arrow indicates a normal tubular epithelial cell and the white asterisk a preserved brush border. (A–C) Magnification 400x; (D–F) scale bars, 5 µm. Images are representative of findings in mice treated with 100 µg, 200 µg, or 400 µg of EHEC O157 OMVs or PBS. (G) Pathology score for tubular epithelial damage was assessed based on the extent of brush border loss, epithelial cell flattening, and vacuolization after 72 h. Data are means ± standard deviations for groups of mice administered the indicated OMV dose or PBS; **** p < 0.0001 for mice treated with 100–400 µg of OMVs compared with mice treated with PBS (one-way ANOVA). (H) Apoptosis shown as <t>DNA</t> laddering in the kidneys of mice treated with 100 µg (lane 2), 200 µg (lane 3), or 400 µg (lane 4) of OMVs for 72 h Lanes 1 and 5, kidneys from mice treated with 5 µg and 25 µg of OMVs, respectively. Lanes 6 and 7, kidneys from mice treated with PBS. Lane 8, kidney treated with 1 µM staurosporine (positive control). Lanes M, molecular size marker (100 bp ladder). The entire original gel is shown in .
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    EHEC O157 OMVs administered to mice by oral gavage cause tubular epithelial damage and apoptosis. Histopathology of paraffin sections stained with hematoxylin–eosin (A–C) and transmission electron microscopy (D–F) of the kidneys from mice treated with 100–400 µg of OMVs (A, B, D, E) and from PBS-treated mice (C, F) . (A) Tubular epithelial cell flattening (black arrows) and intraluminal detached epithelial cells (asterisks). (B) Tubular epithelial flattening (black arrows), extensive vacuolization (blue arrows), detached intraluminal epithelial cells (asterisks). (C) Normal tubules (arrows) and glomeruli in a PBS-treated mouse. (D) Microvacuolization (thin white arrows), macrovacuolization (thick white arrows), and flattening of tubular epithelial cells with a preserved brush border (white asterisks). (E) Macrovacuolization of tubular epithelial cells (red arrows) with lost brush border; the green arrow indicates an <t>apoptotic</t> nucleus. (F) Normal tubule in a PBS-treated mouse; the yellow arrow indicates a normal tubular epithelial cell and the white asterisk a preserved brush border. (A–C) Magnification 400x; (D–F) scale bars, 5 µm. Images are representative of findings in mice treated with 100 µg, 200 µg, or 400 µg of EHEC O157 OMVs or PBS. (G) Pathology score for tubular epithelial damage was assessed based on the extent of brush border loss, epithelial cell flattening, and vacuolization after 72 h. Data are means ± standard deviations for groups of mice administered the indicated OMV dose or PBS; **** p < 0.0001 for mice treated with 100–400 µg of OMVs compared with mice treated with PBS (one-way ANOVA). (H) Apoptosis shown as <t>DNA</t> laddering in the kidneys of mice treated with 100 µg (lane 2), 200 µg (lane 3), or 400 µg (lane 4) of OMVs for 72 h Lanes 1 and 5, kidneys from mice treated with 5 µg and 25 µg of OMVs, respectively. Lanes 6 and 7, kidneys from mice treated with PBS. Lane 8, kidney treated with 1 µM staurosporine (positive control). Lanes M, molecular size marker (100 bp ladder). The entire original gel is shown in .
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    EHEC O157 OMVs administered to mice by oral gavage cause tubular epithelial damage and apoptosis. Histopathology of paraffin sections stained with hematoxylin–eosin (A–C) and transmission electron microscopy (D–F) of the kidneys from mice treated with 100–400 µg of OMVs (A, B, D, E) and from PBS-treated mice (C, F) . (A) Tubular epithelial cell flattening (black arrows) and intraluminal detached epithelial cells (asterisks). (B) Tubular epithelial flattening (black arrows), extensive vacuolization (blue arrows), detached intraluminal epithelial cells (asterisks). (C) Normal tubules (arrows) and glomeruli in a PBS-treated mouse. (D) Microvacuolization (thin white arrows), macrovacuolization (thick white arrows), and flattening of tubular epithelial cells with a preserved brush border (white asterisks). (E) Macrovacuolization of tubular epithelial cells (red arrows) with lost brush border; the green arrow indicates an <t>apoptotic</t> nucleus. (F) Normal tubule in a PBS-treated mouse; the yellow arrow indicates a normal tubular epithelial cell and the white asterisk a preserved brush border. (A–C) Magnification 400x; (D–F) scale bars, 5 µm. Images are representative of findings in mice treated with 100 µg, 200 µg, or 400 µg of EHEC O157 OMVs or PBS. (G) Pathology score for tubular epithelial damage was assessed based on the extent of brush border loss, epithelial cell flattening, and vacuolization after 72 h. Data are means ± standard deviations for groups of mice administered the indicated OMV dose or PBS; **** p < 0.0001 for mice treated with 100–400 µg of OMVs compared with mice treated with PBS (one-way ANOVA). (H) Apoptosis shown as <t>DNA</t> laddering in the kidneys of mice treated with 100 µg (lane 2), 200 µg (lane 3), or 400 µg (lane 4) of OMVs for 72 h Lanes 1 and 5, kidneys from mice treated with 5 µg and 25 µg of OMVs, respectively. Lanes 6 and 7, kidneys from mice treated with PBS. Lane 8, kidney treated with 1 µM staurosporine (positive control). Lanes M, molecular size marker (100 bp ladder). The entire original gel is shown in .
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    EHEC O157 OMVs administered to mice by oral gavage cause tubular epithelial damage and apoptosis. Histopathology of paraffin sections stained with hematoxylin–eosin (A–C) and transmission electron microscopy (D–F) of the kidneys from mice treated with 100–400 µg of OMVs (A, B, D, E) and from PBS-treated mice (C, F) . (A) Tubular epithelial cell flattening (black arrows) and intraluminal detached epithelial cells (asterisks). (B) Tubular epithelial flattening (black arrows), extensive vacuolization (blue arrows), detached intraluminal epithelial cells (asterisks). (C) Normal tubules (arrows) and glomeruli in a PBS-treated mouse. (D) Microvacuolization (thin white arrows), macrovacuolization (thick white arrows), and flattening of tubular epithelial cells with a preserved brush border (white asterisks). (E) Macrovacuolization of tubular epithelial cells (red arrows) with lost brush border; the green arrow indicates an apoptotic nucleus. (F) Normal tubule in a PBS-treated mouse; the yellow arrow indicates a normal tubular epithelial cell and the white asterisk a preserved brush border. (A–C) Magnification 400x; (D–F) scale bars, 5 µm. Images are representative of findings in mice treated with 100 µg, 200 µg, or 400 µg of EHEC O157 OMVs or PBS. (G) Pathology score for tubular epithelial damage was assessed based on the extent of brush border loss, epithelial cell flattening, and vacuolization after 72 h. Data are means ± standard deviations for groups of mice administered the indicated OMV dose or PBS; **** p < 0.0001 for mice treated with 100–400 µg of OMVs compared with mice treated with PBS (one-way ANOVA). (H) Apoptosis shown as DNA laddering in the kidneys of mice treated with 100 µg (lane 2), 200 µg (lane 3), or 400 µg (lane 4) of OMVs for 72 h Lanes 1 and 5, kidneys from mice treated with 5 µg and 25 µg of OMVs, respectively. Lanes 6 and 7, kidneys from mice treated with PBS. Lane 8, kidney treated with 1 µM staurosporine (positive control). Lanes M, molecular size marker (100 bp ladder). The entire original gel is shown in .

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Enterohemorrhagic Escherichia coli O157 outer membrane vesicles administered by oral gavage cause renal tubular injury and acute kidney failure in mice

    doi: 10.3389/fcimb.2025.1704731

    Figure Lengend Snippet: EHEC O157 OMVs administered to mice by oral gavage cause tubular epithelial damage and apoptosis. Histopathology of paraffin sections stained with hematoxylin–eosin (A–C) and transmission electron microscopy (D–F) of the kidneys from mice treated with 100–400 µg of OMVs (A, B, D, E) and from PBS-treated mice (C, F) . (A) Tubular epithelial cell flattening (black arrows) and intraluminal detached epithelial cells (asterisks). (B) Tubular epithelial flattening (black arrows), extensive vacuolization (blue arrows), detached intraluminal epithelial cells (asterisks). (C) Normal tubules (arrows) and glomeruli in a PBS-treated mouse. (D) Microvacuolization (thin white arrows), macrovacuolization (thick white arrows), and flattening of tubular epithelial cells with a preserved brush border (white asterisks). (E) Macrovacuolization of tubular epithelial cells (red arrows) with lost brush border; the green arrow indicates an apoptotic nucleus. (F) Normal tubule in a PBS-treated mouse; the yellow arrow indicates a normal tubular epithelial cell and the white asterisk a preserved brush border. (A–C) Magnification 400x; (D–F) scale bars, 5 µm. Images are representative of findings in mice treated with 100 µg, 200 µg, or 400 µg of EHEC O157 OMVs or PBS. (G) Pathology score for tubular epithelial damage was assessed based on the extent of brush border loss, epithelial cell flattening, and vacuolization after 72 h. Data are means ± standard deviations for groups of mice administered the indicated OMV dose or PBS; **** p < 0.0001 for mice treated with 100–400 µg of OMVs compared with mice treated with PBS (one-way ANOVA). (H) Apoptosis shown as DNA laddering in the kidneys of mice treated with 100 µg (lane 2), 200 µg (lane 3), or 400 µg (lane 4) of OMVs for 72 h Lanes 1 and 5, kidneys from mice treated with 5 µg and 25 µg of OMVs, respectively. Lanes 6 and 7, kidneys from mice treated with PBS. Lane 8, kidney treated with 1 µM staurosporine (positive control). Lanes M, molecular size marker (100 bp ladder). The entire original gel is shown in .

    Article Snippet: Apoptotic DNA was isolated from the pellet using the Apoptotic DNA Ladder Extraction Kit (Assay Genie, Dublin, Ireland) according to the manufacturer’s instructions.

    Techniques: Histopathology, Staining, Transmission Assay, Electron Microscopy, DNA Laddering, Positive Control, Marker